Tempeh vs Natto: Which Fermented Food Is the Better Protein Source?

Last Updated Apr 10, 2025

Tempeh and natto are both excellent fermented soy products, providing high-quality protein with unique nutritional profiles. Tempeh offers a firmer texture and a higher protein content per serving, making it ideal for those seeking a meat substitute rich in essential amino acids. Natto, while slightly lower in protein, contains beneficial probiotics and nattokinase enzyme, supporting digestion and cardiovascular health.

Table of Comparison

Feature Tempeh Natto
Primary Protein Source Soybeans Soybeans
Fermentation Agent Rhizopus mold Bacillus subtilis natto
Protein Content (per 100g) 19g 17g
Digestibility High Moderate to High
Texture Firm, cake-like Sticky, slimy
Flavor Profile Mild, nutty Strong, pungent
Probiotic Benefits Moderate High
Preparation Usage Grilled, stir-fried, baked Raw, mixed with rice, sauces
Vitamin K2 Content Low High

Introduction to Tempeh and Natto

Tempeh and natto are traditional fermented soybean products renowned for their high protein content and unique health benefits. Both serve as valuable plant-based protein sources with distinct fermentation processes and flavors.

  1. Tempeh Production - Tempeh is made by fermenting cooked soybeans with Rhizopus mold, producing a firm, cake-like texture rich in protein and fiber.
  2. Natto Production - Natto is created by fermenting soybeans with Bacillus subtilis, resulting in a sticky texture and a potent flavor with high protein and probiotic content.
  3. Protein Content - Both tempeh and natto provide complete proteins with essential amino acids, making them excellent plant-based protein choices.

Origins and Traditional Fermentation Methods

Tempeh originates from Indonesia and is traditionally made by fermenting cooked soybeans with the Rhizopus mold, creating a firm cake rich in protein and fiber. Natto, a staple of Japanese cuisine, is produced by fermenting soybeans with Bacillus subtilis, resulting in a sticky texture and strong flavor, packed with nattokinase enzyme. Both fermentation methods enhance protein digestibility and bioavailability, reflecting centuries-old cultural practices in their respective regions.

Nutritional Profiles: Protein Content Comparison

Tempeh contains approximately 19 grams of protein per 100 grams, making it a dense source of plant-based protein suitable for vegan diets. Natto offers slightly less protein, around 17 grams per 100 grams, but provides higher levels of certain vitamins and probiotics.

  • Tempeh Protein Density - Rich in complete proteins with all nine essential amino acids, supporting muscle repair and growth.
  • Natto Protein Quality - Contains moderate protein and a significant amount of vitamin K2, aiding bone health and cardiovascular function.
  • Comparative Nutrient Benefits - Tempeh excels in protein quantity while natto provides probiotic benefits, enhancing digestive health.

Amino Acid Composition in Tempeh vs Natto

How does the amino acid composition in tempeh compare to that in natto as protein sources? Tempeh provides a balanced profile rich in essential amino acids such as lysine and leucine, making it an excellent plant-based protein option. Natto contains higher levels of specific amino acids like glutamic acid and arginine, which contribute to its unique nutritional and functional properties.

Digestibility and Bioavailability of Protein

Tempeh contains proteins that are highly digestible due to the fermentation by Rhizopus oligosporus, which breaks down complex proteins into simpler peptides. Natto, fermented with Bacillus subtilis, offers enhanced protein bioavailability through its nattokinase enzyme, which aids in protein absorption.

Tempeh's protein digestibility score (PDCAAS) is high, often ranging around 0.85 to 0.90, making it an excellent plant-based source. Natto provides complementary benefits by improving gut microbiota, which enhances overall protein utilization. The combination of fermentation metabolites in both tempeh and natto contributes to superior nutrient absorption compared to unfermented soy products.

Additional Nutritional Benefits Beyond Protein

Tempeh provides a rich source of prebiotics, including dietary fiber and antioxidants, which support gut health and reduce inflammation. It also contains significant amounts of vitamins B2, B3, and manganese that aid in energy metabolism and oxidative stress reduction.

Natto offers high levels of vitamin K2, essential for bone health and cardiovascular function, along with nattokinase, an enzyme that helps prevent blood clots. This fermented soybean product also contributes beneficial probiotics that enhance digestive health and boost immune function.

Flavor, Texture, and Culinary Uses

Tempeh offers a nutty flavor and firm, chewy texture ideal for grilling or stir-frying, while natto provides a strong, pungent taste with a sticky, slimy texture often enjoyed with rice or in sushi. Both are rich in protein, but tempeh's milder flavor makes it more versatile for various culinary applications compared to natto's distinctive aroma and taste. Fermented soybeans in tempeh support savory dishes, whereas natto's unique texture and flavor appeal primarily to traditional Japanese cuisine.

Suitability for Various Dietary Preferences

Tempeh offers a versatile plant-based protein source suitable for vegetarians and vegans, rich in essential amino acids and dietary fiber. Its firm texture and nutty flavor make it adaptable to various cuisines and dietary preferences, including gluten-free diets.

Natto, while also plant-based, is especially beneficial for those seeking fermented foods with probiotics, supporting digestive health. Its strong flavor and sticky texture may appeal more to those accustomed to traditional Japanese cuisine, limiting its suitability for some dietary preferences.

Health Impacts: Gut Health and Fermented Foods

Tempeh Rich in probiotics from Rhizopus mold fermentation, tempeh supports gut microbiota diversity and improves digestion. It contains high-quality plant protein with essential amino acids and beneficial isoflavones promoting cardiovascular health.
Natto Fermented with Bacillus subtilis, natto is a potent source of nattokinase enzyme that enhances gut enzymatic activity and supports immune function. This traditional Japanese food provides complete protein along with vitamin K2, which aids bone health and cardiovascular protection.

Related Important Terms

Polyglutamic Acid Bioavailability

Tempeh and natto are both fermented soy products rich in protein, but natto contains significantly higher levels of polyglutamic acid, which enhances nutrient absorption and promotes better bioavailability of proteins. Studies indicate that the unique polyglutamic acid in natto not only improves protein digestibility but also supports gut health more effectively than the lower polyglutamic acid content found in tempeh.

Rhizopus Strain Engineering

Rhizopus strain engineering enhances tempeh production by optimizing fungal growth and protein bioavailability, making it a robust source of plant-based protein compared to natto. While natto relies on Bacillus subtilis fermentation to produce vitamin K2 and nattokinase, tempeh engineered with superior Rhizopus strains offers improved texture, digestibility, and amino acid profiles for protein-rich diets.

Bioactive Peptide Yield

Tempeh delivers a higher bioactive peptide yield compared to natto, enhancing its potential health benefits through improved antioxidant and antihypertensive activities. Fermentation by Rhizopus oligosporus in tempeh optimizes proteolysis, increasing the concentration of these functional peptides more effectively than Bacillus subtilis fermentation in natto.

Fermentation Dehulling

Fermentation dehulling significantly influences protein bioavailability in tempeh and natto, with tempeh's Rhizopus fermentation effectively loosening the seed hulls, enhancing digestibility and nutrient absorption. Conversely, natto's Bacillus subtilis-driven fermentation focuses more on humectation and enzymatic activity without fully removing hulls, resulting in a distinct protein profile and functional benefits.

Tempeh Mycelial Protein Enrichment

Tempeh, enhanced through mycelial protein enrichment, offers a superior plant-based protein source with a complete amino acid profile and higher bioavailability compared to traditional natto. This fermentation innovation increases protein content and digestibility, making tempeh an optimal option for sustainable, nutrient-dense diets.

Natto Spore Optimization

Natto, known for its rich Bacillus subtilis natto spores, provides superior protein bioavailability compared to tempeh, which relies on Rhizopus oligosporus fermentation. Optimizing natto spore concentration enhances enzyme activity and nutrient absorption, making it a more efficient plant-based protein source.

Isoflavone Transformation Index

Tempeh exhibits a higher Isoflavone Transformation Index compared to Natto, enhancing the bioavailability of isoflavones such as daidzein and genistein, which contribute significantly to its protein quality. This increased isoflavone conversion in Tempeh fermentation processes leads to greater antioxidant activity and improved nutritional benefits as a plant-based protein source.

Soybean Microstructure Degradation

Tempeh and natto both utilize soybean fermentation but differ significantly in microstructure degradation; tempeh fermentation by Rhizopus mold breaks down complex cell walls, enhancing protein digestibility and nutrient absorption. Natto fermentation with Bacillus subtilis results in partial degradation of soybean protein matrices, increasing bioavailability of certain peptides and nattokinase enzyme activity, which contributes to unique health benefits and improved protein utilization.

Fermented Vitamin K2 Content

Tempeh contains moderate levels of Vitamin K2 (menaquinone), primarily MK-7, due to fermentation by Rhizopus molds, making it a valuable plant-based protein with beneficial bone health properties. Natto, fermented with Bacillus subtilis, boasts exceptionally high Vitamin K2 content, especially MK-7, offering superior cardiovascular and bone benefits compared to tempeh among fermented protein sources.

Tempeh vs Natto for protein sources. Infographic

Tempeh vs Natto: Which Fermented Food Is the Better Protein Source?


About the author.

Disclaimer.
The information provided in this document is for general informational purposes only and is not guaranteed to be complete. While we strive to ensure the accuracy of the content, we cannot guarantee that the details mentioned are up-to-date or applicable to all scenarios. Topics about Tempeh vs Natto for protein sources. are subject to change from time to time.

Comments

No comment yet